Harry Maguire was withdrawn from the England squad after being given a suspended prison sentence in Greece.
Defender Maguire, 27, was named in Southgate’s latest squad earlier on Tuesday, while his trial was ongoing.
He was found guilty of aggravated assault, resisting arrest and repeated attempts of bribery.
He was also given a jail sentence of 21 months and 10 days, which is suspended for three years, after an altercation on Mykonos.
Southgate said withdrawing Maguire was “in the best interests of all parties”.